Direct Detection and Identification of Enteroviruses from Faeces of Healthy Nigerian Children Using a Cell-Culture Independent RT-Seminested PCR Assay
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چکیده
منابع مشابه
Direct Detection and Identification of Enteroviruses from Faeces of Healthy Nigerian Children Using a Cell-Culture Independent RT-Seminested PCR Assay.
Recently, a cell-culture independent protocol for detection of enteroviruses from clinical specimen was recommended by the WHO for surveillance alongside the previously established protocols. Here, we investigated whether this new protocol will show the same enterovirus diversity landscape as the established cell-culture dependent protocols. Faecal samples were collected from sixty apparently h...
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background: generally, sewage exposed water could be potentially contaminated with enteroviruses. for this reason, enterovirus isolation from sewage specimens is one of the most sensitive indicators for virus circulation in the population. we evaluated the icc-rt-pcr and cell culture methods for detection of enteroviruses in tehran sewage system. methods: this research utilized 63 specimens p...
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We have compared in extracts of activated sludge the number of enteroviruses detectable with buffalo green monkey (BGM) cell-cultures versus the number of enteroviral genomes determined by reverse-transcription quantitative real-time PCR (RT-qPCR). In order to find conditions adequate for quantifying enteroviral RNA isolated from (waste)water we have investigated affinity capture of RNA with po...
متن کاملDetection and molecular characterization of enteroviruses in Korean surface water by using integrated cell culture multiplex RT-PCR.
OBJECTIVE To identify waterborne enteric viruses in Korean surface water. METHODS Integrated cell culture (ICC)-multiplex reverse transcription-polymerase chain reaction (RT-PCR) was simultaneously designed to detect coxsackieviruses (CV), polioviruses (PV), and reoviruses (RV). ICC-multiplex RT-PCR and phylogenetic analysis were conducted using 21 total culturable virus assay (TCVA)-positive...
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We investigated the performance of a seminested PCR (snPCR) assay carried out directly from overnight incubated blood culture bottles of 50 newborn intensive care unit (NICU) patients with suspected candidemia and compared these, for sensitivity, specificity and reliability with results from blood cultures. All positive blood cultures (n = 17) yielded positive results for snPCR, which detected ...
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ژورنال
عنوان ژورنال: Advances in Virology
سال: 2016
ISSN: 1687-8639,1687-8647
DOI: 10.1155/2016/1412838